Regulatable and Modulable Background Expression Control in Prokaryotic Synthetic Circuits by Auxiliary Repressor Binding Sites
نویسندگان
چکیده
منابع مشابه
Identification of repressor binding sites controlling expression of tetracycline resistance encoded by Tn10.
The regulatory region controlling the expression of tetracycline resistance and repressor genes contains two nearly identical regions of dyad symmetry. Deletions of this control region were isolated by digestion with S1 nuclease. The ability of these deletions to bind the tet repressor was determined by an in vivo repressor titration assay. The results indicate that repressor specifically binds...
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ژورنال
عنوان ژورنال: ACS Synthetic Biology
سال: 2015
ISSN: 2161-5063,2161-5063
DOI: 10.1021/acssynbio.5b00111